Journal: Nutrients

Article Title: N-3 Polyunsaturated Fatty Acids Decrease the Protein Expression of Soluble Epoxide Hydrolase via Oxidative Stress-Induced P38 Kinase in Rat Endothelial Cells

doi: 10.3390/nu9070654

Figure Lengend Snippet: Effect of antioxidants on DHA, EPA, or 4-HHE-suppression of sEH protein expression. ( A – C ) RAECs were pretreated with N-acetyl- l -cysteine (NAC; 10 mM) for 1 h, and then with DHA (100 µM), EPA (100 µM), or 4-HHE (15 µM) for 48 h. The protein expression of sEH and β-actin was evaluated by western blotting. Each value represents the mean ± SEM. n = 7. ( D ) The protein expression of p38 kinase (p38), phospho-p38 (p-p38), heme oxygenase 1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2), and β-actin (in cells treated with 15 or 30 µM 4-HHE for 48 h) was determined by western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with control. NS, no significant difference.

Article Snippet: Anti-PECAM-1 (M-20) antibody and anti-heme oxygenase 1 (HO-1) antibody were purchased from Santa Cruz (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Effective Nephroprotection Against Acute Kidney Injury with a Star-Shaped Polyglutamate-Curcuminoid Conjugate

doi: 10.1038/s41598-020-58974-9

Figure Lengend Snippet: St-PGA-CL-BDMC treatment prevents key renal cell death and oxidative stress pathways in FA-AKI. Groups of animals were the same as in preceding figures. ( A ) Representative IHC images of 4-Hydroxynonenal (4-HNE) staining in mice with AKI and the corresponding quantification including all mice in each experimental group. St-PGA-CL-BDMC decreased FA-AKI-induced 4-HNE staining. # p < 0.001 vs control; *p < 0.05 vs FA-AKI. Original magnification x200. Scale bar 100 μm. ( B ) Representative western blot detection of cleaved IL-33 and heme oxygenase-1 (HO-1) in total kidney protein extracts from control, AKI or AKI/St-PGA-CL-BDMC mice. St-PGA-CL-BDMC prevented kidney IL-33 processing/activation and upregulation of HO-1 expression. ( C,D ) Quantification of IL-33, # p < 0.001 vs control; *p < 0.05 vs FA-AKI (C) and HO-1, # p < 0.05 vs control; *p < 0.05 vs FA-AKI. ( D ) western blots including the complete set of animals. ( E ) Nqo-1 mRNA expression assessed by q-RT-PCR in kidneys from control mice and in mice with FA-AKI left untreated or treated with ST-PGA-CL-BDMC. # p < 0.001 vs control; *p < 0.05 vs FA-AKI. Results are presented as mean ± SD.

Article Snippet: Primary antibodies were: phospho-MLKL (1/500; ab196436; Abcam, Inc.); cleaved caspase-8 (1/1000, 8592, Cell Signaling Technology); p-cJUN (1/1000, 3270, Cell Signaling); cleaved IL-33 (1/1000; AF3626; R&D Systems), heme oxygenase-1 (HO-1, 1/2000, ADI-OSA-150-D, Enzo) and p65 (1/1000, 8242, Cell Signaling Technology).

Techniques: Staining, Western Blot, Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction